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Image Search Results
Journal: PloS one
Article Title: Identification of novel genes associated with renal tertiary lymphoid organ formation in aging mice.
doi: 10.1371/journal.pone.0091850
Figure Lengend Snippet: Figure 3. Perivascular immune cell clusters in aged mice have characteristics of tertiary lymphoid organs (TLOs). Representa- tive photomicrographs of a perivascular cell cluster from C57L/J mouse serial sections containing (A) Ki67+ proliferating cells (magnification 200x) and (B) peripheral node addressin (PNAd) expressing high endothelial venules (HEVs) (magnification 4006). Insets show higher- power magnifications of the indicated areas. Arrowheads indicate proliferating lymphocytes (A) and PNAd+ HEVs (B). (C) Podoplanin expression on lymphatic endothelial cells in a lymph vessel in the close proximity of a perivascular cell cluster (left panel: magnification 200x, right panel: 400x). (D) Immunofluorescent double labeling for LYVE-1/ podoplanin (upper row) and VEGFR3/podoplanin (bottom row) on C57Bl/6 mouse kidney sections. Abbreviations: i: infiltrate; lv: lymph vessel; v: vein. doi:10.1371/journal.pone.0091850.g003
Article Snippet: Kidney sections were stained for rat anti-mouse CD45 (pan leukocyte marker, clone 30-F11, BD Biosciences), rabbit anti-human CD3 (T cells, DAKO), rat anti-mouse B220 (B cells, tissue culture supernatant from clone RA3-3A1), rabbit anti-human Ki67 (proliferating cells, NCL-Ki67p, Novocastra - Leica Microsystems B.V.), anti-mouse peripheral node addressin [PNAd] (HEVs, clone MECA-79, Biolegend),
Techniques: Expressing, Labeling
Journal: PloS one
Article Title: Identification of novel genes associated with renal tertiary lymphoid organ formation in aging mice.
doi: 10.1371/journal.pone.0091850
Figure Lengend Snippet: Figure 5. Strains with TLO formation are characterized by reduced numbers of lymph vessels. (A) Representative photomicrograph of peri-arteriolar lymph vessels in a C57BLKS/J mouse kidney (left panel). The right panel shows a high-power magnification of the indicated framed area. Arrowheads indicate podoplanin+ lymph vessels. Abbreviations: a: arteriole; g: glomerulus; lv: lymph vessel; v: vein. (B) The number of podoplanin+ lymph vessels was quantified in selected strains based on the relative absence (BALB/cByJ, C3H/HeJ, RIIIS/J, C57BLKS/J [black bars]) and abundance (BTBR T+ tf/J, C57L/J, P/J, LP/J [white bars]) of TLOs at the age of 20 months. Numbers indicated within each bar represent the number of mice analyzed. (C) The mean number of lymph vessels in the 4 strains without TLOs (BALB/cByJ, C3H/HeJ, RIIIS/J, C57BLKS/J [black bars]) and with TLOs (BTBR T+ tf/J, C57L/J, P/J, LP/J [white bars]) was calculated. Strains with TLO formation had overall significantly lower numbers of lymph vessels compared with strains without TLOs (*P,0.05). doi:10.1371/journal.pone.0091850.g005
Article Snippet: Kidney sections were stained for rat anti-mouse CD45 (pan leukocyte marker, clone 30-F11, BD Biosciences), rabbit anti-human CD3 (T cells, DAKO), rat anti-mouse B220 (B cells, tissue culture supernatant from clone RA3-3A1), rabbit anti-human Ki67 (proliferating cells, NCL-Ki67p, Novocastra - Leica Microsystems B.V.), anti-mouse peripheral node addressin [PNAd] (HEVs, clone MECA-79, Biolegend),
Techniques: